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jak1 jak2 inhibitor baricitinib  (ATCC)


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    ATCC jak1 jak2 inhibitor baricitinib
    Jak1 Jak2 Inhibitor Baricitinib, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfex+transfection+reagent/TransfeX+Transfection+Reagent/bio_rxiv__64898__2026__05__20__726679-161-20-13
    Average 99 stars, based on 185 article reviews
    jak1 jak2 inhibitor baricitinib - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: High-throughput in silico screen uncovers key regulators of 3D genome architecture
    Article Snippet: .. The following day, cells in each well were transfected with either EF1A-hJMJD6-T2A-PuroR-CMV-mCherry or EF1A-T2A-PuroR-CMV-mCherry control plasmids using TransfeX Transfection Reagent (ATCC, ACS-4005) according to the manufacturer’s protocol. ..

    Article Title: The First CRISPR-Based Therapeutic (SL_1.52) for African Swine Fever Is Effective in Swine
    Article Snippet: .. Transfection complexes were prepared by mixing an equimolar aliquot of SL_1.52 and ASFV target gene plasmid totaling 250 ng of DNA, along with 0.5 μL TransfeX Transfection Reagent (ATCC, cat# ACS-4005, Manassas, VA, USA) reagent in 50 μL Opti-MEM (Thermo Fisher Scientific, cat# 51985091, Waltham, MA, USA) and incubating for 15 min. ..

    Article Title: TDP-43 toxic gain of function links ALS/FTLD-TDP and Alzheimer’s Disease through splicing
    Article Snippet: .. APEX-containing cassettes were mixed with Opti-MEM I Reduced-Serum Medium (Gibco 31985-047) and TransfeX Transfection Reagent (ATCC® ACS-4005TM) along with piggyBacTM transposase vector and incubated at room temperature for 15-30 minutes. ..

    Article Title: The molecular basis of force selectivity by PIEZO2.
    Article Snippet: Cells were further verified to be free of mycoplasma using the using the MycoAlert Mycoplasma Detection Kit (Lonza). .. The cells were plated onto Matrigel-coated coverslips with embedded gold fiducials, exchanged into a medium containing 250 μM of the click amino acid trans-cyclooct2-en-l-lysine (axial isomer) (SiChem), and transfected with 1 μg of an equimolar ratio of PIEZO expression plasmid and of the tRNA/tRNA synthetase expression plasmids using TransfeX transfection reagent (ATCC). ..

    Article Title: High-throughput in silico screen uncovers key regulators of 3D genome architecture
    Article Snippet: .. Briefly, the sgRNAs were transfected into cells using TransfeX Transfection Reagent (ATCC, ACS-4005) according to the manufacturer’s protocol. ..

    Article Title: The molecular basis of force selectivity by PIEZO2.
    Article Snippet: .. Each well was transfected with 20 pmol of each of the 4 dsiRNAs in 300 μl Optimem with 7.5 μl TransfeX transfection reagent (ATCC). .. After 48 h, the cells were split and plated onto Matrigel-coated coverslips with embedded gold fiducials, exchanged into a medium containing 250 μM of the click amino acid trans-cyclooct-2-en-l-lysine (axial isomer) (SiChem) and transfected a second time with identical amounts of dsiRNA, with the addition of 1.5 μg of an equimolar ratio of the mPiezo2 and the tRNA/tRNA synthetase expression plasmids.

    Article Title: A nucleosome switch primes hepatitis B virus infection.
    Article Snippet: .. Transfections of HepG2 cells were performed with TransfeX Transfection Reagent (ATCC) according tomanufacturer protocol. ..

    Article Title: Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling
    Article Snippet: The mutations were confirmed by sequencing ( ) (Plasmidsaurus, Oxford Nanopore Technology). .. After 18 h of plating on fibronectin-coated glass bottom dishes, TIME cells (for fixed-cell imaging) or TIME-mNGrActin cells (for live-cell SMI-FSM) were transfected with 0.25 μg (/dish) of WT or mutant HaloTag-fused CD36 plasmid (CD36 WT , CD36 G12V , CD36 G16V or CD36 G12V/G16V ) using Transfex transfection reagent (ATCC, ACS-4005). ..

    Control:

    Article Title: High-throughput in silico screen uncovers key regulators of 3D genome architecture
    Article Snippet: .. The following day, cells in each well were transfected with either EF1A-hJMJD6-T2A-PuroR-CMV-mCherry or EF1A-T2A-PuroR-CMV-mCherry control plasmids using TransfeX Transfection Reagent (ATCC, ACS-4005) according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: The First CRISPR-Based Therapeutic (SL_1.52) for African Swine Fever Is Effective in Swine
    Article Snippet: .. Transfection complexes were prepared by mixing an equimolar aliquot of SL_1.52 and ASFV target gene plasmid totaling 250 ng of DNA, along with 0.5 μL TransfeX Transfection Reagent (ATCC, cat# ACS-4005, Manassas, VA, USA) reagent in 50 μL Opti-MEM (Thermo Fisher Scientific, cat# 51985091, Waltham, MA, USA) and incubating for 15 min. ..

    Article Title: TDP-43 toxic gain of function links ALS/FTLD-TDP and Alzheimer’s Disease through splicing
    Article Snippet: .. APEX-containing cassettes were mixed with Opti-MEM I Reduced-Serum Medium (Gibco 31985-047) and TransfeX Transfection Reagent (ATCC® ACS-4005TM) along with piggyBacTM transposase vector and incubated at room temperature for 15-30 minutes. ..

    Article Title: The molecular basis of force selectivity by PIEZO2.
    Article Snippet: Cells were further verified to be free of mycoplasma using the using the MycoAlert Mycoplasma Detection Kit (Lonza). .. The cells were plated onto Matrigel-coated coverslips with embedded gold fiducials, exchanged into a medium containing 250 μM of the click amino acid trans-cyclooct2-en-l-lysine (axial isomer) (SiChem), and transfected with 1 μg of an equimolar ratio of PIEZO expression plasmid and of the tRNA/tRNA synthetase expression plasmids using TransfeX transfection reagent (ATCC). ..

    Article Title: Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling
    Article Snippet: The mutations were confirmed by sequencing ( ) (Plasmidsaurus, Oxford Nanopore Technology). .. After 18 h of plating on fibronectin-coated glass bottom dishes, TIME cells (for fixed-cell imaging) or TIME-mNGrActin cells (for live-cell SMI-FSM) were transfected with 0.25 μg (/dish) of WT or mutant HaloTag-fused CD36 plasmid (CD36 WT , CD36 G12V , CD36 G16V or CD36 G12V/G16V ) using Transfex transfection reagent (ATCC, ACS-4005). ..

    Incubation:

    Article Title: TDP-43 toxic gain of function links ALS/FTLD-TDP and Alzheimer’s Disease through splicing
    Article Snippet: .. APEX-containing cassettes were mixed with Opti-MEM I Reduced-Serum Medium (Gibco 31985-047) and TransfeX Transfection Reagent (ATCC® ACS-4005TM) along with piggyBacTM transposase vector and incubated at room temperature for 15-30 minutes. ..

    Expressing:

    Article Title: The molecular basis of force selectivity by PIEZO2.
    Article Snippet: Cells were further verified to be free of mycoplasma using the using the MycoAlert Mycoplasma Detection Kit (Lonza). .. The cells were plated onto Matrigel-coated coverslips with embedded gold fiducials, exchanged into a medium containing 250 μM of the click amino acid trans-cyclooct2-en-l-lysine (axial isomer) (SiChem), and transfected with 1 μg of an equimolar ratio of PIEZO expression plasmid and of the tRNA/tRNA synthetase expression plasmids using TransfeX transfection reagent (ATCC). ..

    Imaging:

    Article Title: Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling
    Article Snippet: The mutations were confirmed by sequencing ( ) (Plasmidsaurus, Oxford Nanopore Technology). .. After 18 h of plating on fibronectin-coated glass bottom dishes, TIME cells (for fixed-cell imaging) or TIME-mNGrActin cells (for live-cell SMI-FSM) were transfected with 0.25 μg (/dish) of WT or mutant HaloTag-fused CD36 plasmid (CD36 WT , CD36 G12V , CD36 G16V or CD36 G12V/G16V ) using Transfex transfection reagent (ATCC, ACS-4005). ..

    Mutagenesis:

    Article Title: Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling
    Article Snippet: The mutations were confirmed by sequencing ( ) (Plasmidsaurus, Oxford Nanopore Technology). .. After 18 h of plating on fibronectin-coated glass bottom dishes, TIME cells (for fixed-cell imaging) or TIME-mNGrActin cells (for live-cell SMI-FSM) were transfected with 0.25 μg (/dish) of WT or mutant HaloTag-fused CD36 plasmid (CD36 WT , CD36 G12V , CD36 G16V or CD36 G12V/G16V ) using Transfex transfection reagent (ATCC, ACS-4005). ..



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    (a) Representative immunofluorescence images of GM130 and SPCA1 in keratinocytes. Magnification, 63x. Scale bar = 5 μm. SPCA1 +/- keratinocytes transfected with hSPCA1 exhibit increased SPCA1 expression and more compact Golgi morphology. (b) Representative Western blot and (c) quantification of total levels of SPCA1 relative to β-actin loading control. (d-f) Representative fluorescence images (d) and (e-f) quantification of cell area visualized by phalloidin staining after 4 h of recovery in either complete or basal media as described in . Functional rescue of the cell-spreading defect is observed in hSPCA1-transfected SPCA1 +/- keratinocytes, which gain the ability to regrow, after replating, to levels comparable to hTERT control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 20-60 cells per group. (g) Representative images and (h) quantification of FITC-insulin uptake in keratinocytes. hSPCA1 <t>transfection</t> in SPCA1 +/- cells restored FITC-insulin uptake, similar to control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 30-60 cells per group. (i) Representative fluorescence images and (j) quantification of cell spreading capacity of control keratinocytes in presence of Ca 2+ -ATPase agonist CDN1163 (2.5 μM for 4 h) as indicated. Cell spreading in basal medium after CDN1163 treatment was similar to that in complete medium. (k) Golgi morphology, seen by GM130 immunofluorescence in keratinocytes. Golgi fragmentation in hTERT cells was induced using bisphenol. Golgi fragmentation in SPCA1 mutant keratinocytes is largely reversed by CDN1163. (l) Representative fluorescence images and (m) quantification of Mag-Fluo-4 AM fluorescence in SPCA1 +/- cells; hSPCA1-transfected SPCA1 +/- cells (+SPCA1); hSPCA1-transfected SPCA1 +/- cells acutely treated with 5 µM CDN1163 (+SPCA1+CDN1163) or 5 µM Bisphenol (+SPCA1+Bisphenol) for 30 min. Magnification, 40x. Scale bar = 20 μm. Graph represents mean ± SD for n = 30-35 cells per group. Data were evaluated using Ordinary One-way ANOVA analysis. ns = not significant; *P<0.05; ***P<0.001; ****P<0.0001 .
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    ATCC transfex atcc transfection reagent
    (a) Representative immunofluorescence images of GM130 and SPCA1 in keratinocytes. Magnification, 63x. Scale bar = 5 μm. SPCA1 +/- keratinocytes transfected with hSPCA1 exhibit increased SPCA1 expression and more compact Golgi morphology. (b) Representative Western blot and (c) quantification of total levels of SPCA1 relative to β-actin loading control. (d-f) Representative fluorescence images (d) and (e-f) quantification of cell area visualized by phalloidin staining after 4 h of recovery in either complete or basal media as described in . Functional rescue of the cell-spreading defect is observed in hSPCA1-transfected SPCA1 +/- keratinocytes, which gain the ability to regrow, after replating, to levels comparable to hTERT control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 20-60 cells per group. (g) Representative images and (h) quantification of FITC-insulin uptake in keratinocytes. hSPCA1 <t>transfection</t> in SPCA1 +/- cells restored FITC-insulin uptake, similar to control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 30-60 cells per group. (i) Representative fluorescence images and (j) quantification of cell spreading capacity of control keratinocytes in presence of Ca 2+ -ATPase agonist CDN1163 (2.5 μM for 4 h) as indicated. Cell spreading in basal medium after CDN1163 treatment was similar to that in complete medium. (k) Golgi morphology, seen by GM130 immunofluorescence in keratinocytes. Golgi fragmentation in hTERT cells was induced using bisphenol. Golgi fragmentation in SPCA1 mutant keratinocytes is largely reversed by CDN1163. (l) Representative fluorescence images and (m) quantification of Mag-Fluo-4 AM fluorescence in SPCA1 +/- cells; hSPCA1-transfected SPCA1 +/- cells (+SPCA1); hSPCA1-transfected SPCA1 +/- cells acutely treated with 5 µM CDN1163 (+SPCA1+CDN1163) or 5 µM Bisphenol (+SPCA1+Bisphenol) for 30 min. Magnification, 40x. Scale bar = 20 μm. Graph represents mean ± SD for n = 30-35 cells per group. Data were evaluated using Ordinary One-way ANOVA analysis. ns = not significant; *P<0.05; ***P<0.001; ****P<0.0001 .
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    Image Search Results


    (a) Representative immunofluorescence images of GM130 and SPCA1 in keratinocytes. Magnification, 63x. Scale bar = 5 μm. SPCA1 +/- keratinocytes transfected with hSPCA1 exhibit increased SPCA1 expression and more compact Golgi morphology. (b) Representative Western blot and (c) quantification of total levels of SPCA1 relative to β-actin loading control. (d-f) Representative fluorescence images (d) and (e-f) quantification of cell area visualized by phalloidin staining after 4 h of recovery in either complete or basal media as described in . Functional rescue of the cell-spreading defect is observed in hSPCA1-transfected SPCA1 +/- keratinocytes, which gain the ability to regrow, after replating, to levels comparable to hTERT control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 20-60 cells per group. (g) Representative images and (h) quantification of FITC-insulin uptake in keratinocytes. hSPCA1 transfection in SPCA1 +/- cells restored FITC-insulin uptake, similar to control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 30-60 cells per group. (i) Representative fluorescence images and (j) quantification of cell spreading capacity of control keratinocytes in presence of Ca 2+ -ATPase agonist CDN1163 (2.5 μM for 4 h) as indicated. Cell spreading in basal medium after CDN1163 treatment was similar to that in complete medium. (k) Golgi morphology, seen by GM130 immunofluorescence in keratinocytes. Golgi fragmentation in hTERT cells was induced using bisphenol. Golgi fragmentation in SPCA1 mutant keratinocytes is largely reversed by CDN1163. (l) Representative fluorescence images and (m) quantification of Mag-Fluo-4 AM fluorescence in SPCA1 +/- cells; hSPCA1-transfected SPCA1 +/- cells (+SPCA1); hSPCA1-transfected SPCA1 +/- cells acutely treated with 5 µM CDN1163 (+SPCA1+CDN1163) or 5 µM Bisphenol (+SPCA1+Bisphenol) for 30 min. Magnification, 40x. Scale bar = 20 μm. Graph represents mean ± SD for n = 30-35 cells per group. Data were evaluated using Ordinary One-way ANOVA analysis. ns = not significant; *P<0.05; ***P<0.001; ****P<0.0001 .

    Journal: bioRxiv

    Article Title: Insulin-Regulated Actin Dynamics is Disrupted in a Human Keratinocyte Model of Hailey Hailey Disease

    doi: 10.64898/2025.12.15.694174

    Figure Lengend Snippet: (a) Representative immunofluorescence images of GM130 and SPCA1 in keratinocytes. Magnification, 63x. Scale bar = 5 μm. SPCA1 +/- keratinocytes transfected with hSPCA1 exhibit increased SPCA1 expression and more compact Golgi morphology. (b) Representative Western blot and (c) quantification of total levels of SPCA1 relative to β-actin loading control. (d-f) Representative fluorescence images (d) and (e-f) quantification of cell area visualized by phalloidin staining after 4 h of recovery in either complete or basal media as described in . Functional rescue of the cell-spreading defect is observed in hSPCA1-transfected SPCA1 +/- keratinocytes, which gain the ability to regrow, after replating, to levels comparable to hTERT control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 20-60 cells per group. (g) Representative images and (h) quantification of FITC-insulin uptake in keratinocytes. hSPCA1 transfection in SPCA1 +/- cells restored FITC-insulin uptake, similar to control. Magnification, 40x. Scale bar = 10 μm. Graphs represent mean ± SD for n = 30-60 cells per group. (i) Representative fluorescence images and (j) quantification of cell spreading capacity of control keratinocytes in presence of Ca 2+ -ATPase agonist CDN1163 (2.5 μM for 4 h) as indicated. Cell spreading in basal medium after CDN1163 treatment was similar to that in complete medium. (k) Golgi morphology, seen by GM130 immunofluorescence in keratinocytes. Golgi fragmentation in hTERT cells was induced using bisphenol. Golgi fragmentation in SPCA1 mutant keratinocytes is largely reversed by CDN1163. (l) Representative fluorescence images and (m) quantification of Mag-Fluo-4 AM fluorescence in SPCA1 +/- cells; hSPCA1-transfected SPCA1 +/- cells (+SPCA1); hSPCA1-transfected SPCA1 +/- cells acutely treated with 5 µM CDN1163 (+SPCA1+CDN1163) or 5 µM Bisphenol (+SPCA1+Bisphenol) for 30 min. Magnification, 40x. Scale bar = 20 μm. Graph represents mean ± SD for n = 30-35 cells per group. Data were evaluated using Ordinary One-way ANOVA analysis. ns = not significant; *P<0.05; ***P<0.001; ****P<0.0001 .

    Article Snippet: The plasmid with proper insertion was then transfected into an immortalized KC line (N/TERT-2G) using the TransfeX transfection kit (ATCC # ACS4005) in the presence of JAK1/JAK2 inhibitor, baricitinib (10 μg/ml, AchemBlock # G-5743).

    Techniques: Immunofluorescence, Transfection, Expressing, Western Blot, Control, Fluorescence, Staining, Functional Assay, Mutagenesis